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kinC [2019-08-26 10:20:03]
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kinC [2019-08-26 10:20:03]

two-component sensor kinase, phosphorylates Spo0F and Spo0A, part of the phosphorelay, governs expression of genes involved in biofilm formation
Locus
BSU_14490
Isoelectric point
6.22
Molecular weight
48.68 kDa
Protein length
428 aa Sequence Blast
Gene length
1287 bp Sequence Blast
Function
initiation of sporulation
Product
two-component sensor kinase
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,518,333 1,519,619

Phenotypes of a mutant

The protein

Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of Spo0F as part of the phosphorelay, but also direct phosphorylation of Spo0A PubMed
  • mainly active in the younger, outer regions of a colony (with KinD) PubMed
  • phosphorylates Spo0A in response to the presence of surfactin PubMed, this has been refuted PubMed
  • required for initiation of sliding together with KinB PubMed
  • Domains

  • two transmembrane segments
  • PAS domain
  • C-terminal histidine phosphotransferase domain
  • Modification

  • autophosphorylation on a His residue
  • Effectors of protein activity

  • activity is triggered by potassium leakage PubMed, this has been refuted PubMed
  • activity is triggered by polyisoprenoid lipids formed by YisP PubMed
  • activity is stimulated by the interaction with FloT PubMed
  • Structure

  • 3A0R (HK-RR complex from Thermotoga maritima, 28% identity) PubMed
  • Localization

  • cell membrane (Heterogeneous) PubMed
  • co-localizes with FloT in discrete foci in the membrane PubMed
  • the localization of KinC in membrane microdomains depends on FloA and FloT PubMed, this has been refuted PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon
  • Regulation

  • constitutively expressed PubMed
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    Biological materials

    Mutant

  • 1A632 ( kinC::erm), PubMed, available at BGSC
  • BAL393 (kinC::spc)PubMed
  • BKE14490 (kinC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGACCACCTGCCGCTT, downstream forward: _UP4_GACAGCTGAGAGGAGAAAAA
  • BKK14490 (kinC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATGACCACCTGCCGCTT, downstream forward: _UP4_GACAGCTGAGAGGAGAAAAA
  • References

    Reviews

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    Original publications

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